Brain dysfunctions in neurological diseases

Our research has a translational focus on brain dysfunctions in neurological diseases, notably epilepsy and cerebral tumors with an emphasis on the human brain. Most experiments are conducted either in vivo or on postoperative tissues. Our lab studies electrophysiological signals generated by neuronal activity and synaptic interactions between neurons, together with the cellular and synaptic signaling mechanisms at play in brain disorders. This approach permits progress in understanding:

i) The role played by chronic reorganization in the neuronal network signaling during epilepsy, focusing on interneurons / pyramidal cells and chloride / potassium interactions initiating seizures.

ii) Neuronal interactions with lesions such as tumors with a focus on glutamatergic signaling related to glioma cells and neuronal activity

iii) Neuroplasticity induced by focused ultrasound 

iv) Developing innovative electrodes for high resolution recording and brain disease biomarkers identification.

We mainly use electrophysiological recording of the human brain, both in vivo during awake surgery of gliomas and ex vivo from postoperative tissues (patch-clamp, MEA). Human cortex and glioma tissues are cultured in organotypic condition. Tissues are characterized at the histological and metabolite levels. Ultrasound devices are designed for stimulation.

Major publications during the last 5 years.

Animal models and human tissue compared to better understand and treat the epilepsies. Auteurs : Milior G, Morin-Brureau M, Pallud J, Miles R, Huberfeld G Journal : Epilepsia Année : 2023 Pages : 1175-1189 Pubmed ID : 36807867.

Effect of Levetiracetam Use Duration on Overall Survival of Isocitrate Dehydrogenase Wild-Type Glioblastoma in Adults: An Observational Study Auteurs : Pallud J, Huberfeld G, Dezamis E, Peeters S, Moiraghi A, Gavaret M, Guinard E, Dhermain F, Varlet P, Oppenheim C, Chrétien F, Roux A, Zanello M Journal : Neurology Année : 2022 Pages : e125-e140 Pubmed ID : 34675100

Oculomotor artefacts mimic extreme deltabrush EEG features of autoimmune anti NMDA receptor encephalitis. Auteurs : Huberfeld G, Ben Salah A, Pichit P, Psimaras D, Demeret S, Naccache L. Journal : Clinical Neurophysiology Année: 2021 Pages : 1200-1202 Pubmed ID : 33875375

Gamma-aminobutyric acidergic transmission underlies interictal epileptogenicity in pediatric focal cortical dysplasia Auteurs : Blauwblomme T, Dossi E, Pellegrino C, Goubert E, Iglesias BG, Sainte-Rose C, Rouach N, Nabbout R, Huberfeld G Journal : Annals of Neurology Année : 2019 Pages : 204-217 Pubmed ID : 30597612

Pannexin-1 channels contribute to seizure generation in human epileptic brain tissue and in a mouse model of epilepsy. Auteurs : Dossi E, Blauwblomme T, Moulard J, Chever O, Vasile F, Guinard E, Le Bert M, Couillin I, Pallud J, Capelle L, Huberfeld G, Rouach N Journal : Science Translationnal Medicine Année : 2018 Pages : eaar3796 Pubmed ID : 29848662

Address :
INSERM U1266, 102-108 rue de la santé, 75014, Paris

Team leader :
Gilles Huberfeld
Name of co-team leader :

Administrative Contact Name :


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Key words : #Epilepsie, #gliome, #signalisation synaptique, #interneurones, #tissu humain #Epilepsy, #glioma, #synaptic signaling, #interneurons, #human tissue