Dual technological and neuroscientific objective

The research focus of the group has a dual technological and neuroscientific objective. Firstly, it involves advancing all-optical methodologies by integrating single and multiphoton excitation with spatiotemporal wave-front shaping, holographic illumination, compressed sensing, and probe engineering. Secondly, utilizing this unique combination to investigate, at unprecedented precision, neuronal circuits crucial for visual perception.

The Wave-front engineering Microscopy group is an interdisciplinary team composed by physicists, engineers, biophysicists and neurophysiologists.

We have recently demonstrated that scanless two-photon microscopy also provides important advantages for two-photon voltage imaging. Specifically, we have shown that, in combination with temporal focusing, parallel illumination enables high-contrast, high-resolution in vitro and in vivo voltage imaging in densely labelled preparation (mice cortex and zebrafish larvae) expressing GFP and rhodopsin based genetically encoded voltage indicator. 

Our current research activity focuses on advancing even further the development of these technologies and on the use of the developed technologies for the investigation of the mechanisms regulating functional connectivity and signal processing across the main visual pathways in mice, non-human primates and zebrafish larvae. 

Research areas

• Multiphoton scanless optogenetics
• Two-photon holographic endoscopy
• Two-photon scanless voltage imaging
• All-optical circuits manipulation: circuits optogenetics
• Holographic vision restauration
• Dissemination